2021 Latest Design Rt Pcr Calculation - FastKing RT Kit (With gDNase) – TIANGEN

Features

■ High efficiency: The FastKing RT Enzyme is modified with hydrophobic motif, with RT efficiency more than 95%.
■ Sensitive: As low as 1 ng templates can be accurately identified.
■ Resistance: Capable of reverse transcription of complex templates, with perfect resistance to impurities.
■ Flexible: Genomic DNA  removal and reverse transcription was completed separately. Primers were mixed separately in a tube, flexibly to change other primers.

Specification

Type: Gene modified reverse transcriptase, gDNase
Procedures: Two-step (genomic DNA removal and RT)
RT efficiency: >95%
Template: 1 ng- 2 μg
Operation time: ~21 min
Applications: The reverse transcribed cDNA can be used in conventional PCR, Real time PCR, cDNA library construction.

21 min reaction in one-tube

It only takes 21 min to complete the gDNA removal and efficient reverse transcription process in the same tube without replacing the reaction tube and independent DNase I treatment process. Compared with the traditional method that requires 12-step operation and 140 min reaction, it greatly simplifies the operation steps and saves a lot of operation time.

Outstanding quality of King RTase

——Ultra-high reverse transcription efficiency
——Reverse transcription efficiency is over 95%
The general reverse transcriptase has a reverse transcription efficiency of 40-60%, and cDNA yield can be increased by a higher RNA loading amount. King reverse transcriptase can achieve a reverse transcription efficiency of more than 95% due to its unique high affinity for RNA templates. Therefore, subsequent experiments can be satisfied without the need of a large amount of RNA input, which saves RNA and enables high purity and high yield of cDNA.

Easily read through complex templates

——Easily read through high GC and complex templates
Single-stranded RNA has a wide range of complex secondary structure regions due to hydrogen bonding between strands. Ordinary reverse transcriptase may lead to termination of reverse transcription when encountering complex secondary structure, thus unable to successfully complete cDNA synthesis. However, the new generation of King reverse transcriptase has a unique structural domain, which can destroy the hydrogen bond between RNA strands, thus opening the complex secondary structure of RNA and ensuring the smooth reverse transcription.

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