TRNzol Universal Reagent

New upgrade formula for wider sample adaptability.

TRNzol Universal Reagent could be used to isolate total RNA from samples like virus, bacteria, fungus, animal, plant tissue and body fluid with better lysis ability and higher sensitivity. It maintains the integrity of RNA while disrupting cells and dissolving cell components during sample homogenization. RNA isolated by this product can directly serve as templates for downstream detection or other applications.

Cat. No	Packing Size
4992730	100 ml

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Product Detail

Product Tags

Features

■ High flexibility: Wild range of starting sample volume, suitable for large volume sample extraction in a single reaction.
■ High yield: Precipitation method maximize the yield of RNA in sample.
■ Wide use: Suitable for many different samples such as plant and animal tissue, cultured cells, blood, body fluid, etc.
■ Fast operation: Genomic DNA could be obtained within 1 hour..

Specification

Type: Precipitation based
Sample: Virus, bacteria, fungus, animal, plant tissue, cultured cells and body fluid.
Target: RNA
Operation time: ~1 hour
Applications: TRNzol Universal reagent minimizes contamination of impurities such as DNA and proteins in the purified total RNA, and can be directly used for various molecular biology experiments such as Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, cDNA library construction, RT-PCR, real time PCR and high-throughput sequencing.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

Workflow

Method: 30 mg rat liver tissue, 100 mg rice leaves were collected by liquid nitrogen grinding; 1×10⁶ HepG2 cultured cells and 700 μl Saccharomyces Cerevisiae culture medium (OD600=0.9) were collected by centrifugation. 1 ml of TRNzol Universal Reagent from TIANGEN and the relevant products from supplier L and T were added to each aliquot of sample and RNA extraction was performed following the protocols provided by each supplier. The elution volume was 80 μl, 50 μl, 30 μl and 30 μl for the four samples respectively. 3 μl of the eluate was loaded per lane.
MIII: TIANGEN Marker III;
The electrophoresis was conducted at 6 V/cm for 30 min on a 1% agarose.
Results: TIANGEN TRNzol Universal Reagent can extract high purity and good integrity RNA from rat liver, rice leaves, cultured cells and yeast samples, with high efficiency. The RNA quality is comparable to or slightly higher than that of supplier L and T products.

FAQ

Q: Column blockage

A-1 Cell lysis or homogenization not sufficient

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2 Sample amount is too large

---- Reduce the amount of sample used or increase the amount of lysis buffer.

Q: Low RNA yield

A-1 Insufficient cell lysis or homogenization

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2 Sample amount is too large

----Please refer to the maximum processing capacity.

A-3 RNA is not eluted completely from the column

---- After adding RNase-Free water, leave it for a few minutes before centrifuging.

A-4 Ethanol in the eluent

---- After rinsing, centrifuge again and remove the washing buffer as much as possible.

A-5 Cell culture medium is not completely removed

---- When collecting cells, please make sure to remove the culture medium as much as possible.

A-6 The cells stored in RNAstore are not effectively centrifuged

----RNAstore density is greater than the average cell culture medium; so the centrifugal force should be increased. It is suggested to centrifuge at 3000x g.

A-7 Low RNA content and abundance in the sample

---- Use a positive sample to determine if the low-yield is caused by the sample.

Q: RNA degradation

A-1 The material is not fresh

---- Fresh tissues should be stored in liquid nitrogen immediately or immediately put into the RNAstore reagent to ensure the extraction effect.

A-2 Sample amount is too large

---- Reduce sample amount.

A-3 RNase contamination

----Although the buffer provided in the kit does not contain RNase, it is easy to contaminate RNase during extraction process and should be handled with care.

A-4 Electrophoresis pollution

---- Replace the electrophoresis buffer and make sure the consumables and Loading Buffer are free of RNase contamination.

A-5 Too much loading for electrophoresis

---- Reduce the amount of sample loading, the loading of each well should not exceed 2 μg.

Q: DNA contamination

A-1 Sample amount is too large

---- Reduce sample amount.

A-2 Some samples have high DNA content and can be treated with DNase.

----Perform RNase-Free DNase treatment to the obtained RNA solution, and the RNA can be directly used for subsequent experiments after treatment, or can be further purified by RNA purification kits.

Q: How to remove RNase from experimental consumables and glasswares?

For glasswares, baked at 150°C for 4 h. For plastic containers, immersed in 0.5 M NaOH for 10 min, then thoroughly rinsed with RNase-free water and then sterilize to completely remove RNase. The reagents or solutions used in the experiment, especially water, must be free of RNase. Use RNase-free water for all reagent preparations (add water to a clean glass bottle, add DEPC to a final concentration of 0.1% (V/V), shake overnight and autoclave).

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