TIANprep Rapid N96 Plasmid Kit

High throughput, rapid extraction of small amount of plasmid.

The kit adopts the isopropanol precipitation method to obtain plasmid DNA. The kit adopts a 96-well filter plate N and Buffer III, which are specially developed by TIANGEN, and can quickly and efficiently extract high-purity plasmid DNA. It is suitable for rapid preparation of high-throughput plasmids. 1.3 ml of high-copy plasmid bacterial culture can be processed per well, and 96 samples of plasmid DNA are extracted rapidly. The maximum yield per well is 10-20 μg (the yield is related to factors such as plasmid copy number, bacterial state, etc.). Plasmid DNA extracted by the kit can be used in molecular biology experiments such as restriction enzyme digestion, sequencing, library screening, ligation and transformation.

Cat. No:	Packing Size
4992868	4 preps
4992869	24 preps

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Product Detail

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Features

■ Convenient and simple: 96 samples can be extracted in 2-3 hours.
■ Equipped with a unique TIANRed reagent, it can clearly identify the degree of lysis of the sample in the extraction process to ensure high-purity plasmid DNA.
■ High purity: The high-purity plasmid can be directly used directly in downstream experiments.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Workflow

Experimental Example

TIANprep Rapid N96 Plasmid Kit01

	Plasmid DNA was extracted from 1 ml of overnight cultured Escherichia coli (TOP10) broth (LB medium) using TIANprep Rapid N96 Plasmid Kit, and an average of 8-10 μg of high purity pBS plasmid was obtained. 2 µl plasmid was loaded into each well of a 1% agarose gel, and was detected by electrophoresis.
	Plasmid DNA was extracted from 1 ml of overnight cultured Escherichia coli (TOP10) broth (LB medium) using TIANprep Rapid N96 Plasmid Kit, and an average of 8-10 μg of high purity pBS plasmid was obtained. Vector: pBS plasmid; Strain: TOP10. The plasmid was sequenced by ABI. PRISM 3730XL DNA sequencer