SuperReal PreMix Plus (SYBR Green)

Dual-enzyme family star products with outstanding stability and specificity.

SuperReal PreMix Plus is a special reagent for Real-Time PCR developed on the basis of SuperReal PreMix. It is simple and convenient, with unique two-component HotStart DNA polymerase, optimized buffer system with original H-bond factor and ROX packaged separately. Due to the careful optimization of buffer, the generation of primer dimer and nonspecific amplification can be more effectively reduced, ensuring higher amplification specificity and wide template adaptability.

Cat. No	Packing Size
4992214	20 µl×125 rxn
4992215	20 µl×500 rxn
4992248	20 µl×5000 rxn

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Features

■ Strong specificity: The buffer has a unique equilibrium system of K⁺ and NH⁴⁺ and an original H-Bond factor. The reaction system is upgraded and has higher amplification specificity.
■ High sensitivity: Able to quantitatively detect the expression level of transcripts with low abundance.
■ Good repeatability: The experimental results are stable and have good repeatability.
■ Simple and fast: Only template, primer and ddH₂O need to be added in the premix to carry out real-time PCR reaction.
■ ROX correction: ROX dyes packaged separately are more flexible to use and can ensure more accurate results.
■ Widely applicable: It is widely applicable to experiments such as gene expression analysis and nucleic acid detection by SYBR Green method on various real-time PCR instruments such as ABI, Stratagene, Roche, Bio-Rad and Eppendorf.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

	The dual-enzyme system ensures stable and efficient amplification In some reaction systems, compared with the traditional single-enzyme amplification system, the dual-enzyme automatic regulation system has stronger fluorescence value and higher amplification efficiency.
	Comparison of Dual HotStart enzyme automatic regulation system and traditional single enzyme system In the amplification experiment of hard to amplify template, the H-bond factor buffer system of SuperReal PreMix Plus has stronger reaction specificity and better repeatability than the common buffer system.