SuperReal PreMix Plus (Probe)

Dual-enzyme probe quantitative reagent with stable performance.

SuperReal PreMix Plus (Probe) adopts a chemical and antibody modified two-component hot-start DNA polymerase to carry out PCR amplification. The double-hotstart polymerases makes SuperReal PreMix maintain the best DNA polymerase activity throughout the whole PCR reaction process. With careful optimization of Buffer system, it has the characteristics of accurate quantification, high amplification efficiency, good repeatability and wide credible range.

Cat. No	Packing Size
4992290	20 µl×125 rxn
4992291	20 µl×500 rxn
4992305	20 µl×5000 rxn

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Product Detail

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Features

■ Dual-enzyme advantage: Dual-enzyme hot-start system can ensure strong stability and more accurate data.
■ Wide linear detection range: The linear detection range can be up to 107.
■ High sensitivity: Low abundance templates such as viruses and microorganisms can be detected.
■ Strong amplification capability: Stronger fluorescence signal.

Specification

Type: Chemical and antibody-modified hot-start DNA polymerase, probe
Linear range: 100-107
Operation time: ~40 min
Applications: Probe-based qPCR for gene detection on DNA or cDNA samples from various biological areas.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

	Wide linear detection range The product has a wide linear detection range. It can detect templates as low as 1 fg/μl for lamda DNA, with high amplification efficiency, good repeatability and excellent linear relationship.Use lamda DNA as template, dilute 7 gradients by 10 times (concentration from 1 ng/μl to 1fg/μl) for PCR detection.
	Strong amplification capability, more standard amplification curve and higher sensitivity The amplification fluorescence signal is strong (amplification capability is strong), with a more standard amplification curve and high sensitivity. It can accurately and quantitatively detect low-concentration templates, while the detection signal of relevant product from Supplier T is weak, resulting in low sensitivity, which may lead to undetectable low-concentration templates and false negative results.Detect lectin gene with soybean gDNA (100-0.01 ng/μl) as template and compare with relevant product from Supplier T.
	Wide adaptability of instruments It has been proven by multiple tests that the product is widely applicable to experiments such as gene expression analysis and nucleic acid detection by adopting a Probe method on various real time PCR instruments such as ABI, Stratagene, Roche, Bio-Rad, Eppendorf, etc.