RNAstore Reagent

Features

■ Storage conditions: This kit can be stored for 1 week at room temperature, 1 day at 37℃, and at least 1 month at 4℃. For tissue samples, immerse at 4℃ overnight, and transfer to -20℃ or -80℃ for long-term storage.
■ Repeated freezing and thawing: Tissue frozen at -20℃ or -80℃ can be freeze-thawed for 20 times without affecting the quality of RNA extraction.
■Downstream applications: After removing the sample from the RNAstore Reagent, total RNA can be extracted by TIANGEN’s TRNzol, RNAprep Pure, RNAsimple reagents and kits.

Important Notes

■ RNAstore is only suitable for fresh tissue samples.
■ RNAstore is not suitable for plant tissue samples.
■ The amount ratio of the tissue samples and RNAstore Reagent should be at least 1:10 (eg for 100 mg tissue, at least 1 ml of RNAstore is required).
■ The thickness of each side of the sample should not exceed 0.5 cm to ensure that the RNAstore can penetrate quickly into the tissue.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

Material: 15 mg Rat liver tissue Method: 0.5 g rat liver tissues (stored in RNAstore Reagent) were stored at 37 ℃ , room temperature and 4 ℃ respectively. The total RNA from 15 mg rat liver tissue samples stored at different temperatures were isolated using the TRNzol Reagent (Cat. no. 4992730). Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 100 μl eluates were loaded per lane. C (positive control): tissue sample directly stored at -80℃ . The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose.

Material: 15 mg Rat liver tissue Method: 0.5 g rat liver tissues (stored in RNAstore Reagent) were freezethawed for 5, 10, 15 and 20 times respectively. The total RNA from 15 mg rat liver tissue samples freeze-thawed for different times were isolated using the TRNzol Reagent (Cat. no. 4992730). Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 100 μl eluates were loaded per lane. C (positive control): tissue sample directly stored at -80℃ . 5, 10, 15, 20: the freeze-thawed times of samples. The electrophoresis was conducted at 6 V/cm for 30 min on 1% agarose.

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