RNAprep Pure Plant Plus Kit

For purification of total RNA from polysaccharides & polyphenolics-rich plant samples.

The RNAprep Pure Plant Plus Kit (Polysaccharides & Polyphenolics-rich) is a silicon matrix purification RNA extraction kit developed for multiple plant samples with polysaccharides and polyphenolics. It is supplied with Buffer SL with superb lysis ability. High-purity total RNA without gDNA can be extracted from the fleshes of bananas, watermelons, apples, pears, the tubers of sweet potatoes, potatoes, as well as the leaves of cotton, rose, alfalfa, rice and white pine needles within 1 hour.

Cat. No	Packing Size
4992239	50 preps

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Product Detail

Product Tags

Features

■ Targeted: It is specially formulated for plant samples that are difficult to extract, such as polysaccharides and polyphenolics-rich plants. The process is more optimized, and the results are reliable.
■ Efficient removal of gDNA: High-efficient DNase I is supplied for rapid removal of gDNA on the column.
■ Easy and fast: RNA extraction experiments can be completed within 1 hour.
■ Safe and low toxicity: no toxic organic reagents such as phenol and chloroform are needed.

Applications

This kit can be directly used for various molecular biology experiments such as RT-PCR, Real-time PCR, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, and cloning, etc.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Example

Total RNA was extracted from 100 mg fleshes of banana, watermelon, apple and pear, tubers of sweet potato and potato, leaves of cotton, rose, alfalfa, rice and white pine needles respectively using RNAprep Pure Plant Plus Kit. 4-6 μl of 30 μl eluates were loaded per lane.
M: TIANGEN Marker III;
The electrophoresis was conducted at 6 V/cm for 30 min on a 1% agarose.
Results: RNAprep Pure Plant Plus Kit can extract high purity, high yield and good integrity total RNA from the polysaccharides & polyphenolics-rich plant samples.

FAQ

Q: Column blockage

A-1 Cell lysis or homogenization not sufficient

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2 Sample amount is too large

---- Reduce the amount of sample used or increase the amount of lysis buffer.

Q: Low RNA yield

A-1 Insufficient cell lysis or homogenization

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2 Sample amount is too large

----Please refer to the maximum processing capacity.

A-3 RNA is not eluted completely from the column

---- After adding RNase-Free water, leave it for a few minutes before centrifuging.

A-4 Ethanol in the eluent

---- After rinsing, centrifuge again and remove the washing buffer as much as possible.

A-5 Cell culture medium is not completely removed

---- When collecting cells, please make sure to remove the culture medium as much as possible.

A-6 The cells stored in RNAstore are not effectively centrifuged

----RNAstore density is greater than the average cell culture medium; so the centrifugal force should be increased. It is suggested to centrifuge at 3000x g.

A-7 Low RNA content and abundance in the sample

---- Use a positive sample to determine if the low-yield is caused by the sample.

Q: RNA degradation

A-1 The material is not fresh

---- Fresh tissues should be stored in liquid nitrogen immediately or immediately put into the RNAstore reagent to ensure the extraction effect.

A-2 Sample amount is too large

---- Reduce sample amount.

A-3 RNase contamination

----Although the buffer provided in the kit does not contain RNase, it is easy to contaminate RNase during extraction process and should be handled with care.

A-4 Electrophoresis pollution

---- Replace the electrophoresis buffer and make sure the consumables and Loading Buffer are free of RNase contamination.

A-5 Too much loading for electrophoresis

---- Reduce the amount of sample loading, the loading of each well should not exceed 2 μg.

Q: DNA contamination

A-1 Sample amount is too large

---- Reduce sample amount.

A-2 Some samples have high DNA content and can be treated with DNase.

----Perform RNase-Free DNase treatment to the obtained RNA solution, and the RNA can be directly used for subsequent experiments after treatment, or can be further purified by RNA purification kits.

Q: How to remove RNase from experimental consumables and glasswares?

For glasswares, baked at 150°C for 4 h. For plastic containers, immersed in 0.5 M NaOH for 10 min, then thoroughly rinsed with RNase-free water and then sterilize to completely remove RNase. The reagents or solutions used in the experiment, especially water, must be free of RNase. Use RNase-free water for all reagent preparations (add water to a clean glass bottle, add DEPC to a final concentration of 0.1% (V/V), shake overnight and autoclave).

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