Magnetic Tissue/Cell/Blood Total RNA Kit

Extract RNA from various samples such as tissue cell blood with high throughput.

Magnetic Tissue/Cell/Blood Total RNA Kit adopts magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality total RNA. The product can be perfectly matched with Kingfisher Flex96 and TGuide S32/S96 Automated Nucleic Acid Extractors. If high-throughput automated extraction is required, please contact TIANGEN for the integration solution.

Cat. No Packing Size
4992740 50 preps

Product Detail

Product Tags

Features

■ Easy and fast: Ultrapure total RNA can be obtained within 60 min.
■ High throughput: It can meet the requirements of manual extraction as well as batch extraction on various high-throughput platforms.
■ Safe and non-toxic: No reagent such as phenol/chloroform is needed.

Specification

Type: Magnetic beads type extraction.
Sample: Tissues, cells and blood.
Target: Total RNA
Starting volume: 5-20 mg, not exceed 1×107, 0.1-1.5 ml
Operation time: 60 min
Downstream applications:  RT-PCR/RT-qPCR, NGS library construction, etc.

All the products can be customized for ODM/OEM. For details, please click Customized Service(ODM/OEM)

Experimental Examples

Experimental Examples Total RNA was extracted from 20 mg rat liver with TIANGEN Magnetic Tissue/Cell/Blood Total RNA Kit and the relevant products from other suppliers. 5 μl of 200 μl eluate was loaded to 1% agarose gel electrophoresis, 6 V/cm.
Conclusion: The extraction yield, purity and stability of TIANGEN Magnetic Tissue/Cell/Blood Total RNA Kit are better than that of other suppliers.
Experimental Examples Total RNA was extracted from 20 mg of rat kidney with TIANGEN Magnetic Tissue/Cell/Blood Total RNA Kit in TIANGEN TGuide S32 or Thermo Kingfisher Flex96 respectively. 5 μl of 200 μl eluate was loaded to 1% agarose gel electrophoresis, 6 V/cm. Marker: TIANGEN D15000 DNA Marker.
Conclusion: Magnetic Tissue/Cell/Blood Total RNA Kit has good extraction yield and purity in different automated extractors.

FAQ

Q: Column blockage

A-1 Cell lysis or homogenization not sufficient

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2 Sample amount is too large

---- Reduce the amount of sample used or increase the amount of lysis buffer.

Q: Low RNA yield

A-1  Insufficient cell lysis or homogenization

---- Reduce sample usage, increase the amount of lysis buffer, increase homogenization and lysis time.

A-2  Sample amount is too large

----Please refer to the maximum processing capacity.

A-3 RNA is not eluted completely from the column

---- After adding RNase-Free water, leave it for a few minutes before centrifuging.

A-4 Ethanol in the eluent

---- After rinsing, centrifuge again and remove the washing buffer as much as possible.

A-5 Cell culture medium is not completely removed

---- When collecting cells, please make sure to remove the culture medium as much as possible.

A-6 The cells stored in RNAstore are not effectively centrifuged

----RNAstore density is greater than the average cell culture medium; so the centrifugal force should be increased. It is suggested to centrifuge at 3000x g.

A-7 Low RNA content and abundance in the sample

---- Use a positive sample to determine if the low-yield is caused by the sample.

Q: RNA degradation

A-1  The material is not fresh

---- Fresh tissues should be stored in liquid nitrogen immediately or immediately put into the RNAstore reagent to ensure the extraction effect.

A-2  Sample amount is too large

---- Reduce sample amount.

A-3  RNase contamination

----Although the buffer provided in the kit does not contain RNase, it is easy to contaminate RNase during extraction process and should be handled with care.

A-4  Electrophoresis pollution

---- Replace the electrophoresis buffer and make sure the consumables and Loading Buffer are free of RNase contamination.

A-5  Too much loading for electrophoresis

---- Reduce the amount of sample loading, the loading of each well should not exceed 2 μg.

Q: DNA contamination

A-1  Sample amount is too large

---- Reduce sample amount.

A-2  Some samples have high DNA content and can be treated with DNase.

----Perform RNase-Free DNase treatment to the obtained RNA solution, and the RNA can be directly used for subsequent experiments after treatment, or can be further purified by RNA purification kits.

Q: How to remove RNase from experimental consumables and glasswares?

For glasswares, baked at 150°C for 4 h. For plastic containers, immersed in 0.5 M NaOH for 10 min, then thoroughly rinsed with RNase-free water and then sterilize to completely remove RNase. The reagents or solutions used in the experiment, especially water, must be free of RNase. Use RNase-free water for all reagent preparations (add water to a clean glass bottle, add DEPC to a final concentration of 0.1% (V/V), shake overnight and autoclave).


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